Device
Part:BBa_K4062025:Design
Designed by: Benjamin Bateman Group: iGEM21_WLC-Milwaukee (2021-10-16)
lactose regulated bla, CAP mutation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2914
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1982
Design Notes
The CAP binding site is inactivated by a deletion, so its regulatory effects will not occur on any downstream genes.
Source
Sequences from the Escherichia coli K12 genomic library from biocyc.org