Device

Part:BBa_K4062025:Design

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-16)


lactose regulated bla, CAP mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1982


Design Notes

The CAP binding site is inactivated by a deletion, so its regulatory effects will not occur on any downstream genes.


Source

Sequences from the Escherichia coli K12 genomic library from biocyc.org

References